Vol. 1, Issue 3 (2016)
Larvicidal Activity of Clausena Anisata Oils and Extracts Anopheles Gambie Larvea
Author(s): Moses A Ollengo, John M Vulule, Josephat C Matasyoh
Abstract: Clausena anisata extracts are known to show bioactivity against several diseases here we report their larvicidal activity. Dried and ground 600 g of aerial parts of the plant C. anisata were sequentially extracted with solvents of increasing polarity, five fractions were obtained. The oils were obtained by hydro-distillation in a modified Clevenger-type of fresh whole plant. The essential oils (4.25% w/w) were dried over anhydrous sodium sulphate. The oils and active nonvolatile fractions were characterized by spectroscopic techniques. The major compounds in the oils were monoterpenes whereas the nonvolatile fractions had sesquiterpenes. The bioassays were performed with third instar larvae of A. gambiae s.s. The LC50 were 75.96 and 2095.46 mg l-1 for the oils and the active ethyl acetate fraction respectively. Their corresponding LC99 were 256.80 and 4438.75 mg l-1 respectively. Application of these extracts to larval habitats may prove useful in malaria and mosquito management programmes.