In the present study, an attempt was made to investigate pharmacognostical and phytochemical screening of Delonix regia seeds. The pharmacognostical parameters studied were macroscopy, microscopy, physicochemical analysis and chromatography. The phytochemical screening revealed that the presence of secondary metabolites such as carbohydrates, sterols, triterpenoids, saponins and fixed oils in Delonix regia seeds. All the parameters were studied according to the WHO and Pharmacopoeial guidelines.