Anti cataract activity of Methanolic extract of Ixora coccinea flower leaves on dexamethasone induced cataract by using isolated goat lens
N Jyothirmai, R Vamsi, SK Abul Kalam, V Prem Kumar
Objective: To investigate anti cataract activity of Methanolic extract of Ixora coccinea flower on Prednisolone induced cataract by using isolated goat lens. Methods: Anti cataract activity is done by using isolated goat lens. Goat lens were divided into four groups. Group I lens were incubated in artificial aqueous humour (normal control).Group II lens were incubated with dexamethasone 10mg (toxic control).Group III and IV lens were incubated with dexamethasone and methanolic extract of (50µg and 100µg) Ixora coccinea flower and subjected to photographic evaluation for opacity, lens were homogenized by using tris phosphate buffer and sodium, potassium, total protein and catalase concentrations were determined Results: The grades of opacity was 0,3,1 and 1 in group I,II,III and IV respectively. The present study showed higher total proteins (P < 0.05 at all concentration) and K+ ions (P<0.05 at all concentration) whereas lower concentrations of Na+ ions (P<0.05 at all concentration) with MEIC treated groups. The level of Catalase was found to be less in experimentally induced cataract lenses as compared to normal control group. The lenses treated with EEAS showed significant rise in enzyme level suggesting maintenance of antioxidant enzyme integrity. Conclusion: The Present investigation suggests that Methanolic extract of Ixora coccinea flower effectively prevent the cataractogenic condition. Thus, the goat lens model and dexamethasone induced cataract model could be used for testing of various anti cataract agents.