International Journal of Research in Pharmacy and Pharmaceutical Sciences

International Journal of Research in Pharmacy and Pharmaceutical Sciences






ISSN: 2455-698X


International Journal of Research in Pharmacy and Pharmaceutical Sciences
International Journal of Research in Pharmacy and Pharmaceutical Sciences
Vol. 5, Issue 5 (2020)

Phytochemical, pharmacognostical estimation and antioxidant potental of nerium indicum mill. stem extracts


Shrinivas Sarje, Aparna Suryavanshi, Vivek Shinde, Zeeshan Ahmed, Mohzib Shaikh

The objective of this study was to carry out phytochemical and pharmacognostic and quantitative evaluation of Stem of nerium indicumm mill. Nerium is a distinct genus of evergreen coniferous tree from the family Apocynaceae, containing only one species, Nerium indicum (Kaner). Stem and powdered microscopy revealed useful diagnostic features. Phytochemical testing of varies extracts revealed the presence of glycosides, flavonoids, tannins, phenolic compounds, carbohydrates etc., which was further confirmed by TLC. The total phenolic and flavonoid content of plant extracts was expressed as Gallic acid equivalents and as rutin equivalents respectively. Total phenolic content and flavonoid contents was found to be more in ethanolic extract of stem as compared to ethyl acetated extract respectively. In-vitro Antioxidant activity study indicates that ethyl acetated extract has the more antioxidant effect (98.89% inhibition of free radicals at 100µg/ml) as compared to ethanol extract. Accordingly safe experimental dose was calculated as ≤ 200mg/kg & was used accordingly for further screening of extracts.
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