International Journal of Research in Pharmacy and Pharmaceutical Sciences

Authors
Srivastava AK, Gupta A, Naseer A

Abstract
The main objective of this study was to identify the antioxidant potential of a Polyherbal formulation. It involves preparation of a poly-herbal formulation by preparing different polar solvent extract of the four plants namely, Mucuna Pruriens (MP), Bacopa monnieri (BM) & Withania somnifera (WO), were extracted using Soxhlet Apparatus. Extracts obtained by different solvents (ethanol, ethyl acetate, chloroform, and n-hexane) were tested for total antioxidant capacity, DPPH against Scavenging activity, and total polyphenol and flavonoid contents. The antioxidant potential (DPPH Scavenging activity) of the above formulation was compared with other similar marketed formulations. The screening done by using DPPH scavenging activity showed that the free radical scavenging effect of Polyherbal formulation (PHF) at concentration 100 μg/ml (i.e. 92. 43%) reveals maximum percentage inhibition of free radicals. The effects mentioned at concentration 25 μg/ml and 50 μg/ml were found to be more antioxidant efficacy than reference standard drug. Finally the results concluded that, all the used solvents are able to extract compounds having high antioxidant activity except as regards n- hexane, when compared to ascorbic acid.