Magnolia officinalis is a woody medicinal plant belonging to the Magnolia family and Chinese traditional medicine. The present study comprises phytochemical screening of different extracts of the seeds of M. officinalis and quantitative analysis of amino acids in the seeds of M. officinalis. The present study concluded that the seeds contains variety of phytoconstituents. Phytochemical studies on the extracts of seeds showed presence of proteins and amino acid, steroid and terpene, carbohydrates, alkaloids, phenolic and tannins, flavonoids, saponins, fats and oils. This information may be further useful for isolation of various compounds from seeds for treatment of diseases in human beings. Moreover, 17 amino acids were identified in the seeds, of which 7 were essential. The total bound and free amino acids in the seeds of M. officinalis was 8065.0 mg/100g and total free amino acids in the seeds was 93.3 mg/100g. The seeds contains abundant amino acids and can be used as potential food additives.