Painrnand inflammation can be eradicated by using various drugs. Herbal drugs can bernopted to control pain, as they have few side effects. This study was done torncompare anti-inflammatory, analgesic and antipyretic of aqueous extractrnof T. foenum-graecum sprouts (fenugreek) on albino mice withrndiclofenac sodium as a standard of reference. A microbial screening wasrnconducted. Initially, seeds were collected, soaked in water, sprouts formedwerern then the active constituents was extracted with water.

Thernanalgesic activity of aqueous extract of T. foenum-graecum sproutsrn(fenugreek) on albino mice was found using hot plate method and acetic acidrninduced writhing method. Complete blood count (CBC) was also done.

Thernanimals were pretreated with diclofenac sodium (10mg/kg b.w.) i.p and T. foenum-graecumrnsprouts (200mg/kg b.w.) i.p for 4 days and latency time on hot platernwithout licking or jumping was checked at 30 minutes after the administrationrnof last dose. T. foenum-graecum sprouts (200mg/kg b.w.)i.p differedrnsignificantly from the control (P<0.01)rnThe latency time of fenugreek sprouts had decreased by 12.06% In the aceticrnacid writhing test maximum analgesic activity was observed in the mice treatedrnwith diclofenac sodium (10mg/kg b.w.) i.p. CBC was also done for all thernanimals.

Arnfurther study was done to compare antipyretic activity of aqueous extractrnof T. foenum-graecum sprouts (fenugreek) on albino mice withrndiclofenac sodium. Temperature was measured by rectal route using digitalrnthermometer. The animals were pretreated with diclofenac sodium (10mg/kg b.w.)rni.p and T. foenum-graecum sprouts (200mg/kg b.w.) i.p for 4 days.Pyrexiarn was induced by subcutaneous administration of 20 % brewer’s yeastrnsuspension (10ml/kg). Twenty four hours after injection of yeast, bodyrntemperature was recorded. Maximum temperature reduction was observed in animalsrntreated with fenugreek sprout extract.

Thernacute anti-inflammatory activity of T. foenum-graecum sproutsrn(200mg/kg b.w.) i.p. was measured plethysmo graphically using carrageenan asrninflammatory agent and comparing with diclofenac sodium (10mg/kg b.w.) i.p asrnreference standard. T. foenum-graecum sprouts (200mg/kg b.w.) hasrnsignificant difference with control (P<0.05)rnat the 2^nd, 4th, 6th hour.

Finallyrnan antimicrobial screening was done. It was found that the sprouts had no antifungalrnactivity and was having antibacterial effect on gram negative bacteria.