Diabetes mellitus is the most common endocrine disorder that impairs glucose homeostasis resulting in severe diabetic complications including retinopathy, angiopathy, nephropathy, and neuropathy causing neurological disorders due to perturbation in utilization of glucose. The aqueous leaf extract of Passiflora ligularis were investigated for its anti-diabetic effect in albino rats after induction of diabetes (alloxan). Oral administration of aqueous extract of P. ligularis to diabetic rat for 30 days resulted in a decrease in blood glucose. Aqueous extract of P. ligularis leaf can decrease the blood glucose and reduce the oxidative stress by removing free radicals in diabetes.