Comparative antimicrobial study using nanocopper, nanoselenium and anti-metabolite produced from curved Halomonas variabilis-PRS2 strain isolated from Port Said salt Marsh, Egypt
Extremotolerant isolatePRS2 and antimetabolite producer, identified as Gram negative curved haloalkalophiles mesophilic strain, isolated from Port Said salt marsh, Egypt. Strain PRS2 grows at temperatures ranges from 15–37°C, pHs 6.0–10.5 and salinities range 3.0–28.5 % NaCl (w/v). Non-carbohydrate fermented except little growth with glucose and fructose. Euclidean distance was 90% between isolate PRS2 verity and Halomonas variabilis- Phylum Proteobacteria Delta proteo bacteria and genus Halomonas. Effect of different pH values and NaCl concentration on antimetabolite production using E.coli organism were carried out. Highly antimetabolite production under 12%NaCl and between pHs ranges from 8 to 11. Inhibitions clearing zone (ICZ) was measured using concentrated antimetabolite alone and conjugated with copper and selenium nanoparticles. The ICZ values were done against various seven gram positive bacterial strains, six gram negative strains and seven fungal strains. Antimicrobial activity showed excellent results against all the clinical pathogens. Generally though the effect of selenium nanoparticles pluse antimetabolite were found to be excellent more pronounced against various pathogenic microbe ranged from25.6±12mm to 33± 1.5mm. Followed by the inhibitory effect of selenium single ranged from23.0 ± 1.2mm to26.4 ± 0.63mm.The effect of copper nanoparticles pluse antimetabolite against microbial pathogen ranged from 17.6±0.58mm to 25.6±0.63mm. While nanocopper particles single and antimetabolite single exhibits moderate results against microbial pathogen. Ranged from 13.4+1.5mm to21.4±0.63mmand17.3± 0.78mm to24.6± 0.82mm respectively.
Rawhia Arafa. Comparative antimicrobial study using nanocopper, nanoselenium and anti-metabolite produced from curved Halomonas variabilis-PRS2 strain isolated from Port Said salt Marsh, Egypt. International Journal of Research in Pharmacy and Pharmaceutical Sciences, Volume 2, Issue 1, 2017, Pages 29-41