The aim of present work was to develop a simple, accurate and precise area under curve (AUC) spectrophotometric method for estimation of cefixime trihydrate and validation according to ICH Q2 (R1) guideline. The area selected for estimation of cefixime trihydrate was between 262.80 to 303.60 nm. The method represented correlation coefficient (R²= 0.999) at concentration rang 5-25 μg/ml. The Satiafactory value of percent relative standard deviation for the intraday and inter-day precision indicates that method was precise. The recovery of the cefixime trihydrate was found upto 100.12 %. The developed methods can be successfully applied in routine work for the estimation of cefixime trihydrate in its pharmaceutical dosage form.