Medicinal plants have been reported as richest bioresource of drugs in modern medicines, folk medicines, food supplements, pharmaceuticals intermediates, nutraceuticals, chemical entities for synthetic drugs and traditional medicine system. The aim of our present study was designed to quantify the presence of phytochemicals constituents in the different fresh and dried parts of
G. polycaulon in hexane solvents. The phytochemical analysis was carried out quantitatively in the fresh and dried hexane extracts of Gnaphalium polycaulon and confirmed by TLC analysis with standard procedure. All dried hexane extracts showed significant results than fresh hexane extracts due to the presence of major phytoconstituents in Gnaphalium polycaulon. These findings confirmed that Gnaphalium polycaulon plant have medicinal property for the treatment of various diseases.