Withania somniferais the best rejuvenating agent. Withania somnifera commonly known as “Ashwagandha”, and is belongs to family Solanaceae. The present study comprises physico-chemical and phytochemical evaluation of different extracts of Withania somnifera by using different standard methods. The evaluation of physico chemical parameters was carried out by the determination of ash values, extractive values and moisture content. Whereas phytochemical investigation was carried out to estimate the presence of carbohydrates, glycosides, flavonoids, tannins, phytosterols and phenolic compounds in different extracts of Withania somnifera. Results revealed the presence of carbohydrates, alkaloids, saponons, phytosterols, flavonoids, fats and oils. The present investigation will helpful in Assessing the quality and purity of a crude drug and laying down pharmacopoieal standards for Withania somnifera.