International Journal of Research in Pharmacy and Pharmaceutical Sciences

International Journal of Research in Pharmacy and Pharmaceutical Sciences


International Journal of Research in Pharmacy and Pharmaceutical Sciences
International Journal of Research in Pharmacy and Pharmaceutical Sciences
Vol. 2, Issue 3 (2017)

Simultaneous UV spectrophotometric estimation of Acebutalol hydrochloride and hydrochlorothiazide in bulk and combined tablet dosage form


Pawar Seemarani, Jadhav Santosh, Tamboli Ashpak, Shaikh Ajij, Mali Seeta

There is not a singal analytical methods appeared in the literature for combined estimation of Acebutalol Hydrochloride and Hydrochlorothiazide in tablets dosage form. Attempts were made to develop a simple, precise and accurate Simultaneous UV spectroscopic method of Acebutalol Hydrochloride and Hydrochlorothiazide in bulk and Sectrazide tablet dosage form by using simultaneous equation method. UV spectrophotometric method was developed and validated as per ICH guidelines using methanol as mobile phase. Acebutalol Hydrochloride and Hydrochlorothiazide individually follows the Beer-Lamberts law over concentration range 3-18 μg/ml and 1-6 μg/ml, regression of coefficient was found to be r2=0.9999 and r2=0.9999 respectively. The percentage recovery was found in the range of 98% to 102% at three different levels. The proposed method was successfully applied for the determination of Acebutalol Hydrochloride and Hydrochlorothiazide in tablets dosage form as per ICH guidelines the result of the analysis were validated statistically and were found to be satisfactory.
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How to cite this article:
Pawar Seemarani, Jadhav Santosh, Tamboli Ashpak, Shaikh Ajij, Mali Seeta. Simultaneous UV spectrophotometric estimation of Acebutalol hydrochloride and hydrochlorothiazide in bulk and combined tablet dosage form. International Journal of Research in Pharmacy and Pharmaceutical Sciences, Volume 2, Issue 3, 2017, Pages 70-74
International Journal of Research in Pharmacy and Pharmaceutical Sciences