Highly porous 3Dimensional (3D) scaffold becomes a promising alternative approach for tissue repairing. Tissue engineering (TE) is an interdisciplinary field involving principles of engineering and biological sciences to regenerate new tissue and organs using cells and scaffolds. Functional biomaterial research has focused on the development and improvement of scaffolding which has played an important role in the therapeutic approach in the current and future medicine. Scaffolds are one of the decisive factors for tissue engineering. It consists of natural polymers which have recently been developed more quickly and have gained more popularity. These include chitosan, a copolymer derived from the alkaline deacetylation of chitin. Expectations for use of these scaffolds are increasing as the knowledge regarding their chemical and biological properties expands, and new biomedical applications are investigated. Due to their different biological properties such as being biocompatible, bio adhesive, biodegradable, and bioactive, they have given the pattern for use in tissue engineering for repair and/or regeneration of different tissues including skin, bone, cartilage, nerves, liver and muscle. Here we highlight recent advances in the development of chitosan-based scaffolds with enhanced tissue regeneration capability.