Sensitive and accurate spectrophotometric methods have been developed for the assay of oxybutynin hydrochloride (OXB) in bulk drug and pharmaceutical preparations. The proposed methods are based on oxidation reaction of OXB with a known excess of cerium (IV) ammonium sulphate (CAS) as an oxidizing agent in acid medium followed by determination of unreacted oxidant by adding a fixed amount of dye e.g. amaranth (AM), rhodamine 6G (Rh6G) and indigo carmine (IC) followed by measuring the absorbance at 520, 530 and 610 nm, respectively. The effect of experimental conditions was studied and optimized. The beer’s law was obeyed in the concentration ranges of 2.0-12, 1.0-10, and 2.0-14 μg mL^-1 using AM, Rh6G and IC dyes, respectively with a correlation coefficient ≥ 0.9994. The calculated molar absorptivity values are 1.8347 × 10⁴, 3.285 × 10⁴ and 2.5097 × 10⁴ L mol^-1 cm^-1 using AM, Rh6G and IC dyes, respectively. The limits of detection and quantification were reported. Intra-day and inter-day accuracy and precision of the methods have been evaluated. No interference was observed from the additives. The proposed methods were successfully applied to the assay of OXB in tablets preparations and the results were statistically compared with those of the reported method by applying Student’s t-test and F-test. The reliability of the methods was further ascertained by performing recovery studies using the standard addition method.