The study carried out among 30 patients suffering with dental caries, attending the out patients Department of Conservative Dentistry, MGPGI, Pondicherry, and 30 controls attending the students of MGPGI the results revealed as the oral microflora from the dental caries showed prevalence of Streptococcus mutans (50%) for 41-50 age groups and 42.2% for 30-40 age groups. Streptococcus viridans are more prevalence for 41-50 age groups 40%. Klebsiella pneumoniae (21.4%) are more prevalence for 30-40 age groups. Neisseria catarrhalis (50%) are more prevalence for 51; 60 age groups. The oral microbial flora of the normal population showed the prevalence of Neisseria catarrhalis to a tune of 75%. The other aerobic organism showed as Streptococcus viridians isolated (64%) from normal controls. The other most prevalence microflora Streptococcus mutans (43%), E.coli (26%), Enterococcus spp (20%), Pseudomonas aeruginosa (7%), Staphylococcus aureus (40%).