Diabetes a dreadful disease is found in all parts of the world and is becoming a serious threat to mankind health. The results of the present study reveals a strong antidiabetic activity of the aqueous extract of Embilica Officinalis, Trigonella foenum-graecum, Azaridicta Indica, Ocimum Sanctum Syzygium Cumini for polyherbal syrup in the present antidiabetic study of aqueous extraction of poly-herbal syrup were tested in In-vitro model method in present study. Poly-herbal syrup prepared successfully using decoction method and for the characterization study of poly-herbal syrup color, Odour, Taste, pH, Viscocity were checked and compared with standard. In-vitro studies for anti diabetic activity using Glucose bound test and Glucose diffusion test were performed. In the present work, hypoglycemic activity of the aqueous extracts of Embilica Officinalis, Trigonella Foenum Graecum, Azadirect Indica, Ocimum Sanctum, Syzygium Cumini are used to formulate a poly-herbal anti-diabetic syrup which showed a significant reduction of glucose concentration in in-vitro studies. Many plants exhibit antidiabetic activity and anti-inflamatory property.