The present study deals with the investigation to assess the preliminary phytochemical constituents and in vitro antibacterial activity of Centella asiatica. The different solvent extracts such as hexane, chloroform, ethyl acetate and ethanol were used for extracting bioactive compounds present in leaf. The phytochemical tests were revealed the presence of alkaloids, total phenols, glycosides, terpenoids, steroids, flavonoids, tannins, saponins and reducing sugars. The ethanolic extract showed maximum yield of bioactive compounds than the other solvent extracts. Proteins, antheroquinones and amino acids were absent in all the extracts. Ethanol and ethyl acetate extracts were yielded maximum percentage on nine bioactive compounds than hexane and chloroform extracts yielded six compounds rich. The results were validating the traditional uses of this plant in treatment of various diseases. Five gram-positive (Bacillus cereus, Bacillus subtilis, Streptococcus cremoris, Streptococcus fecalis, Staphylococcus aureus) and five gram-negative (Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Proteus vulgaris, Shigella dysentriae) bacterial strains were tested. The maximum in vitro antibacterial activity were recorded 30.7 mm in Bacillus cereus at 250 µl concentration followed by S. aureus (28.1 mm), S. cremoris (27.2 mm), Streptococcus fecalis (24.6 mm), B. subtilis (22.3 mm), E. coli (20.6 mm), P. aeruginosa (19.2 mm), Shigella dysentriae (18.3 mm) and Proteus vulgaris (17.5 mm) and least inhibition was observed in Klebsiella pneumonia (16.2 mm). Moderate activity was observed in ethyl acetate and chloroform extracts. Minimum activity was observed in hexane extract at different concentration tested. Compared to synthetic antibiotic Ampicillin (50 mg), solvent extracts showed significant antibacterial activity. The present findings revealed that the presence of bioactive compounds and their activity against selected pathogenic bacteria. These results will support to the traditional knowledge of the medicinal plants to the local users and these plants used as therapeutic agents for treat several diseases.