Anthocephalus cadamba is one of such Medicinal Plant that has been mentioned in many Indian medicinal literatures. It is crucially significant as it has the largest number of phytochemicals and secondary metabolites having pharmacological and biological properties. The present work deals with development and standardization of phytochemical screening for quantification of stem bark extract of medicinal plant of Anthocephalus cadamba (Roxb.) Miq. The scientific parameter is necessary to identify the exact plant material and to find its quality and purity. The present study deals with various preliminary phytochemical screening of various successive extracts such as qualitative chemical analysis. The alkaloidal content of stem bark has been determined. These studies indicated the possible information for correct identification and standardization of this plant material.