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VOL. 3, ISSUE 1 (2018)
New spectrophotometric method for determination of Aripiprazole in pure form and pharmaceutical formulations using ceric (IV) ammonium Sulphate
Authors
Ragaa El Sheikh, Ayman A Gouda, Lobna A Sadek
Abstract
Four simple, sensitive and accurate spectrophotometric methods have been developed for the determination of aripiprazole in pure form and pharmaceutical formulations. The methods are based on the oxidation of aripiprazole by a known excess of ceric(IV) ammonium sulphate (CAS) in acid medium followed by determination of unreacted CAS by adding a fixed amount of methylene blue (MB), amaranth (AM), indigo carmine (IC), and methyle orange (MO) dyes followed by measuring the absorbance at 664, 520, 610 and 510 nm, respectively. The experimental conditions affecting the reaction were studied and optimized. The beer’s law was obeyed in the concentration ranges of 0.2-4.0, 0.5-6.0, 0.2-5.0 and 0.5-8.0 μg mL-1 using MB, AM, IC and MO methods, respectively with a correlation coefficient ≥ 0.9990. The calculated molar absorptivity values are 9.616 × 104, 3.377 × 104, 5.504 × 104 and 3.747 × 104 L mol-1 cm-1 using MB, AM, IC and MO methods, respectively. The limits of detection and quantification are also reported. Intra-day and inter-day precision and accuracy of the methods have been evaluated. The methods were successfully applied to the assay of aripiprazole in tablets and the results were statistically compared with those of the reference method by applying Student’s t-test and F-test. No interference was observed from the common tablet excipients. The accuracy and reliability of the methods was further ascertained by performing recovery studies using standard-addition method.
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Pages:259-267
How to cite this article:
Ragaa El Sheikh, Ayman A Gouda, Lobna A Sadek "New spectrophotometric method for determination of Aripiprazole in pure form and pharmaceutical formulations using ceric (IV) ammonium Sulphate". International Journal of Research in Pharmacy and
Pharmaceutical Sciences, Vol 3, Issue 1, 2018, Pages 259-267
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