Lactobacillus species are group of bacteria that are generally used to prepared probiotic foods and stimulate the growth of plants. In the present study, bacteria were isolated from curd on MRS agar medium. The isolates were identified by cultural, morphological and biochemical characterization. Lactobacillus isolates were found to be rod shaped, gram positive, non-motile. In case of seed germination on the growth of Bengal gram (Cicer arietinum), Peas (Pisum sativum) and Mustered seeds(Bassica hirta) crop plants, it was noted that enhanced growth rate was obtained; hence Lactobacillus sp. can be used as bioinoculant.