A simple, precise, accurate and cost effective UV-visible spectrometry method and validation for water soluble vitamin folic acid in the pellet formulation was developed according to ICH Q2 (R1) guideline. The absorption maxima of folic acid was found to be 283nm. The method represents correlation coefficient (R² =0.999) at concentration range of 1-3.5 μg/ml. The validation of method was carried out using linearity, accuracy and precision values. The percent relative standard deviation of inter-day and intra-day precision range (0.884-0.713) & (0.627-0.142) respectively shows the method was precise. The recovery of folic acid was found to be 99.66 - 99.36 %. This validated precise UV- visible spectrometry method can be applied for the estimation of folic acid with respect to the determination of assay content for the solid dosage form.