In the present study, drugs were targeted to colon by using combination of pH reliant polymers used to make Aceclofenac pellets coated with multiple layers of swelling polymer and time dependent polymer. The drug solubility was maximum at alkaline or weak acidic than strong acidic conditions for Aceclofenac. Multiparticulate approach to achieve colon targeted Aceclofenac pellets containing Hydroxypropyl cellulose as swelling layer to retard the drug release in a sustained manner and Ethylcellulose as time dependent outer coating, to protect the upper GI environment conditions. Both polymers were coated on the drug absorbed beads in conventional coating pan. Furthermore, in-vivo gastrointestinal transit of drug irrespective of their type of polymer used to delay the released at pre-designed time intervals. Also physical presence and quantitative analysis of drug pellets at small intestine colon are estimated. Presume that the drug is well scattered and absorbed at intestine and colonic areas from the measured length and amount of drug analysed. It was studied for combinations of polymers could achieve budding applications as a colon–specific drug delivery device with added gain for scale up.