The present investigation was carried to find out the phytochemical and biochemical constituents present in Carica papaya. The ethanolic extract of Carica papaya seed contains carbohydrate, protein, flavonoids, alkaloids, saponin, steroids, and glycosides. The chloroform extract of Carica papaya seed contain carbohydrate, protein, amino acid, alkaloid, tannin, and saponin. The acetone extract of Carica papaya seed contain carbohydrate, protein, amino acid, and alkaloid. The moisture content in dry seeds of Carica papaya is 9.5% and ash content 15%. The physicochemical parameters such as ash value and determination of moisture loss was used to determine the quality and purity of a crude drug. The biochemical analysis on Carica papaya revealed 18.75 mg of carbohydrates, 7.28 mg protein and 16.87 mg starch. The extracts of Carica papaya seeds have great medicinal and nutrient values which could be used for treating many ailments such as ringworm, psoriasis, vermifuge, liver cirrhosis, abortifacient and maintenance of the body. All the nutrients of papaya improve cardiovascular system, protect against heart diseases, heart attacks, strokes and prevent colon cancer. Carica papaya is a source of inspiration for novel drug compounds as plants derived medicines have made significant contribution towards human health.