Introduction:Euphorbia pilosa is much branch, milky latex bearing tall herb commonly used to treat bronchitis, Purgative, emetic, fistulous sores and asthma-related problems. The plants were collected in the month of September 2015 from Kalamuni (Munsiari) near Pithoragarh, location of Kumaon Himalayas. Methods:The plant Euphorbia pilosa including leaves, stem, and flowers were extracted by hydro distillation method for 6 hours using Clevenger apparatus. The hydro-distilled essential oil of Euphorbia pilosa has been examined by means of gas chromatography-mass spectrometry (GC-MS). Results: Phytochemical investigation of whole plants of Euphorbia pilosa led to the isolation and identification of essential oil. Separation was achieved by Elite-5MS fused capillary column. The mass spectra were compared with the spectra of known components stored in the NIST and WILEY databases for compound identification. Forty-six chemical constituents were identified. The major constituents were Phytol (5.75%), n-Pentadecanal (5.12%), n-Pentadecane (4.02 %), δ-Terpineol (3.31) and Tricos-(9Z)-ene (3.24%). Conclusion: The results data obtained in the present study suggest that essential oil possesses strong medicinal activities can be utilized for treatment of diseases.