In this study the thrombolytic activity and cytotoxic activity of Goniothalamus Sesquipedalis was evaluated. The plant is associated to the Annonaceae family and locally used for tumors, boils and buboes treatment. The thrombolytic property and the cytotoxic activity of Goniothalamus Sesquipedalis was evaluated by using the aqueous, methanolic, ethanolic, ethyl acetate, chloroform and their cyclohexane soluble partitioning materials and also extracts of Goniothalamus Sesquipedalis. As standard for the thrombolytic activity evaluation Streptokinase was used and % of clot lysis was 86.31 which is very high. For Cytotoxic activity Vincristine sulphate was used as standard and IC50 value of the methanolic extracts was 25.19µg/ml which was comparatively best.