To formulate and evaluate papaya leaves and euphorbia hitra by using different sweetening agents like sucrose, maltose, sucralose and saccharin etc. Medicinal plants such as Carica papaya, and Euphorbia hitra, is an ever green medicinally useful tree that tolerates various stress of the desert. Medicinal plants have been a variable source of natural activity phytochemical constituents that play an important role in the treatment of many human diseases. In the present study, leaves of Carica papaya, euphorbia hitra was by its Anti-bacterial activity the qualitative phyto-chemical analysis and bacterial potential. The extraction was done by using soxhlation process. The prepared poly herbal syrup were evaluated such as color, odour, taste pH, specific gravity, viscosity. The Anti-bacterial activity was evaluated by cup plate method against different pathogenic Gram negative and Gram-positive bacteria. The abstractive yield was maximum in methanol extraction with papaya and euphorbia hitra, leaves etc., The prepared were evaluated for the polyherbal syrup by In-vitro permeation studies using dialysis membrane, among all the 6 formulations F6 formulation was shown 84.8% cumulative drug release within 8 hours.