Salmonella has been one of the most prevalent reported etiological agents in fresh-produce-associated outbreaks of both human and animal infections. The principal objective of this research study was to develop a suitable rapid and simple PCR-based protocol for routine analysis of Salmonella spp. in fresh vegetables and forages. Fifty-two (24 vegetables and 28 forages) samples collected from different farms in Zarqa, Jordan, where recycled wastewater from Khirbet Al-Samra Wastewater Treatment Plant serves as a source of irrigation water were examined by standard microbiological techniques (SMTs). The same samples were analyzed directly by PCR. The results for both methods (SMTs and PCR) were the same for Salmonella spp. The results proved that the PCR technique was applicable to detect Salmonella spp. in fresh vegetable and forage samples in less time and with high accuracy. The combination of both methods (PCR & SMTs) can give a more effective and more accurate profile of the prevalence of Salmonella spp. in fresh vegetable and forage related samples.