This review article gives knowledge of about High-Performance Thin Layer Chromatography, HPTLC-based analytical method development and its applications in food industry, in accordance to practical evaluation. Among the modern Analytical tools, HPTLC is a powerful analytical method equally suitable for qualitative and quantitative analytical tasks. HPTLC is on TLC, but with enhancements that tend to increase the resolution of the compounds to be separated and allows both quantitative and qualitative analysis of the compounds.