Genus Mitragyna is distributed in tropical and subtropical regions of Africa and Asia. Different species of Mitragyna are widely used in ethnomedicine against malaria, fever, worms, diarrhea and cough. Mitragyna speciosa is a natural remedy for fatigue. Mitragyna ciliate, Mitragyna inermis and Mitragyna stipulosa have been used traditionally against hypertension, inflammation, rheumatism, gonorrhea and bronchpulmonary diseases. Mitragyna africanus is used traditionally against mental diseases. In this study stem barks of Mitragyna inermis were extracted with 95% ethanol and the crude extract was fractionated over silica gel plates to yield a chromatographically pure flavonoid – compound I. The structure of the isolated compound has been partially characterized by its spectral data (UV and ¹HNMR).