Medicinal plants are considered to be rich source of therapeutic agents for prevention of diseases. Betel and Miswak are medicinal plants which are being used in Indian system of medicine for their effect on various diseases. The extracts of Miswak and Betel were tested against different bacteria and moulded to form as a unit dosage in the form of jelly. The aim of the study is to observe the antibacterial activity of extracts against different bacterial strains and the formulation and evaluation of the herbal jelly. The quality of the herbal jelly was evaluated with different parameters, physical appearance (colour, odour, taste, texture and shape), pH, average weight, and total microbial count.