Methanol extracts obtained from Andrographis paniculata and Ocimum sanctum have been investigated for their antimicrobial activity with disc diffusion method. Antimicrobial activity was determined with pathogens such as Gram positive bacteria Staphylococcus aureus and Gram negative bacteria Aeromonas hydrophila, Escherichia coli, by the disc diffusion method. The extracts of Andrographis paniculata and Ocimum sanctum were impregnated with empty sterile disc in the concentration of 6mg/disc, 3mg/disc1.5mg/disc0.75mg/disc. The extracts of Andrographis paniculata and Ocimum sanctum had strong antimicrobial activity against the Gram-positive bacteria but slightly lesser activity was recorded against the Gram-negative bacteria used in this study. Zone of microbial inhibition for gram positive, Gram negative bacteria were significantly higher in the extract of Andrographis paniculata (6mg/disc) than Ocimum sanctum (6mg/disc) and commercially available antibiotic in the concentration of (10µg).