There has been continuous research for herbal remedies to combat malaria which is gradually becoming resistant to the available orthodox drugs. These drugs pose a risk of toxicity to humans and are becoming more expensive in Nigeria. This research is aimed at investigating the antimalarial potential of the herbal combinations containing Harungana madasgariensis, Costus afer, and Citrus auritifolia. The Herb was prepared by the maceration of the stem bark of by H. madagascariensis at g/ml of distilled water. The stem of Costus afer was debarked by peeling and then pounded in a wooden mortar to extract its juice and filtered. The juice of Citrus aurantifolia was obtained by squeezing the fruit and filtering. The three plants H.madagascariensis, C.afer, and C.aurantifolia were combined in a ratio of 100ml:100ml:10ml respectively in a bottle and preserved at 4^oC. Acute toxicity of the herbal combination was assessed using Lorke method. The antimalarial assay was evaluated in vivo using Chloroquine sensitive Plasmodium berghei induced mice. The herbal preparation was screened for Phytochemical constituents, Thin layer chromatography and Fourier transform Infrared analysis were carried out for characterization. The acute toxicity showed LD50 of 1300mg. The curative antimalarial study showed that the highest dose (260mg/kg) exhibited a significant reduction of the parasitemia level against the control group. The Phytochemical screening of the herbal combination showed the presence of tannins, cardenolides, deoxy sugars and triterpenes. The FTIR showed the presence of free OH, aliphatic CH, and C=C bonds. Thus, it could be concluded that the Herbal combination of H. madagascarensis, C. afer, and C. auritifolia is safe at 1300mg/kg and has a significant curative effect at the highest dose (260mg/kg) and its effect could be attributed to the functional groups present in the combination.