Phytochemical screening was carried out on the methanol extract of Solanum dubium leaf. It revealed the presence of flavonoids, tannins, saponins, sterols and alkaloids. Flavonoids are known to exhibit biological activities, including antiallergic, antiviral, anti-inflammatory, and antimicrobial properties. Flavonoids are potent water- soluble antioxidants and free radical scavengers, which prevent oxidative cell damage and have anticancer activity. In this study the methanol extract of Solanum dubium leaf was evaluated for antioxidant activity. The extract showed significant antioxidant activity compared to the positive control – propyl gallate.