One of the most crucial aspects of living a healthy life is human health. Although many diseases are found in human being, however the anxiety disease, which is a psychiatric disorder, is most commonly found in human being. To treat this anxiety disease, many herbal plants plays very significant role. The purpose of this study was to evaluate the anxiolytic action of hydro-alcoholic extract of laves of the Mimosa Pudica plant in mice animals. To evaluate the anxiolytic action of this plant in mice, various animal models like elevated plus maze, light and dark test, social interaction test and hole board test were used. To obtained hydro alcoholic extraction of leaves of Mimosa Pudica plant, soxhlet extraction method was used, in which air dried leaves were used. Preliminary phytochemical investigations of abve extraction were also carried out. The standard drug diazepam (1 mg/kg) was used as anxiolytic agent and the doses of hydro-alcoholic extract of leaves of Mimosa Pudica plant were p.o. 200, and 400 mg/kg. Results recommended that hydro alcoholic extract of leaves of Mimosa Pudica at 400 mg/kg dose produced antianxiety effect consequences nearly much like diazepam. The data indicates that the hydro alcoholic extract of Mimosa Pudica leaves has more anti-anxiety action than the control. Further studies are needed to identify the anxiolytic mechanism(s) and the phytoconstituents responsible for the observed central effects of the hydro-alcoholic extract of Mimosa Pudica.