The relationship between humans and microorganisms has been around for a long time. Some bacteria help to achieve symbiotic balance by providing protective and stabilizing effect on the human body. Recent microbial and pathogenic bacteria invade humans tissues,causing harm to the human body and endangering his Life. Human's discovered cure for it many bacteria were discovered due to there adverse shape.The use of antibiotics was found to have determinant effect on the normal and useful microorganisms of human body. Many types of antibiotics are used not only for therapeutic purposes but also in other industries such as agriculture and animals husbandry. Adequate understanding of the mechanism of action of antibiotics and hence the importance of protecting the human health care delivery system. Recent molecular biological approaches have greatly helped to understand the mechanism of action in this paper by emphasizing the classification of antibiotics and molecular senses and Reiterates optimal use of antibiotics.