Wound healing activity of ethanolic extract of leaves of Xanthium strumarium was studied by Chorioallantoic membrane (CAM) model (In vitro) in 09-day old embryonated chicken eggs. The extract also promoted angiogenesis as evidenced by the CAM model. The result suggested that ethanolic extract of Xanthium strumarium possess significant wound healing potential in the normal wound. The preliminary phytochemical screening of the successive extracts of selected plant species viz., methanol aqueous extract confirmed the presence of carbohydrates, glycosides, alkaloids, phenolics and tannins, saponins, flavonoids. The phytochemical findings of the study confirm the presence of plant phenolics, flavonoids and other secondary metabolites which are currently of growing interest owing to their functional properties in promoting human health. Flavonoids and other plant phenolics act as remedies in the treatment of stress-related ailments and as dressings for wounds, cuts, rheumatism etc.In present investigation, objective of the study was to extract the Xanthium strumarium L. leaves and evaluate its phytochemical and wound healing potential along with antioxidant property. The ethanolic extract of Xanthium strumarium leaves had shown activity from slight to mark which was dose dependent high dose of extract (400mg) showed the marked increases in blood vessels.