Introduction: Blepharis maderaspatensis is used as a diuretic medicine as a folklore plant. Aim & Objective: The aim of the research study is to evaluate the single dose diuretic activity of seed powder and hydro-alcoholic extract of the Utigan. Materials & Methods: The wistar albino rats were divided into 6 different groups containing 6 animals per group. The normal control group, ref. std. group (10 mg/kg, po), Utigan powder at low and high dose (67.5mg/kg and 135mg/kg) and Utigan extract at low and high dose (100mg/kg and 200mg/kg po) respectively. 1 hour after the administration of drug animals were kept in metabolic cages and urine of 6hrs and 24 were collected and volume, and electrolytes were evaluated. Result & Observation: Utigan powder at high dose and extract at low dose significantly increase urine volume at 24 hrs. Utigan powder at low and high dose significantly increase the excretion of Na+ and K+ through urine and have high diuretic index. In the conclusion it is revealed that Utigan powder at both doses shows diuretic activity and extract have less diuretic activity when compared with normal control group. Conclusion: This study shows the diuretic activity of B. maderaspatensis possibly mediated through loop diuretic effects, which rationalizes its medicinal use as a diuretic.