Carrageenan is a polysaccharide extracted with water or alkaline solvents from red algae species (Rhodophyceae). Carrageenan has a variety of uses in the pharmaceutical industry, such as stabilizers, thickening, gelling agents, emulsifiers, and binders. Every year, the Indonesian carrageenan industry has good growth, and it necessitates efforts to establish efficient manufacturing procedures to produce high-quality carrageenan. This study aimed to determine the characteristics of Eucheuma spinosum carrageenan resulting from the drying process using the Freeze Drying method with different freezing temperatures. The sample of red algae (Eucheuma spinosum) came from the Sumenep, Madura sea. The quality characteristics tested include yield, moisture content, sulfate content, gel strength, viscosity, and ash content. Based on the results, the different freezing temperatures in the freeze-drying method did not affect the yield of carrageenan and the quality parameters of the gel strength standard. The ash content results were in the range of 35.90% - 38.70%, where the results met the carrageenan quality standard set by FAO of 15-40%.