Aim: Aim of the study was to establish analytical method development and validation for Amoxicillin capsules according to ICH guidelines. The chromatographic separation was achieved by using Syncrosis C8 (100x4.6mm, 3μ) column by applying isocratic elution using the mobile phase consist of 0.1% formic acid in water and acetonitrile (90:10), detection was carried at the 230nm by employing 0.8ml/min flow rate. The retention time was found to be 2.897 min, 2.808min for generic samples and 2.897 min, 2.798 min for branded samples respectively. For the developed method linearity range was obtained by UPLC method in between 10-1000μg/ml. Robustness, accuracy and precision were evaluated for the developed method and results were found to be within the limit and the result were reproducible. Based on these results, it was concluded that developed method was precise and accurate for the analytical method development and validation for Amoxicillin capsules. This method can be helpful for in-vitro studies in future days.