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International Journal of
Research in Pharmacy and Pharmaceutical Sciences
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VOL. 8, ISSUE 3 (2023)
Identification of compound content and antioxidant activity test of ethanol extract of Stachytarpheta jamaicensis (l.) vahl. leaf abts method
Authors
Imrawati Imrawati, Yuri Pratiwi Utami, Ade Ainun Insani
Abstract
Stachytarpheta jamaicensis (L.) Vahl leaves contains secondary metabolites in the form of flavonoids, which can be used as a source of antioxidants. All flavonoids have been reported to undergo some form of degradation with exposure to physico-chemical environmental factors. This study aims to identify the compound content and determine the antioxidant activity of horse whip leaf extract. Identification of compound content by TLC method and antioxidant activity in reducing ABTS radicals using a UV-VIS spectrophotometer which is then expressed in IC50 value. Extraction by maceration method placed at room temperature, namely 30oC. The results of the identification of the compounds contained in the horse whip leaf extract were positive for flavonoids, saponins, tannins and terpenoids. ABTS method antioxidant activity with IC50 value 39.41 ppm is included in the very strong antioxidant category because it is < 50 ppm compared to Vitamin C which IC50 value is 4.07 ppm. It can be concluded that the very strong activity of the ethanol extract of horse whip leaves is thought to be due to its compound content, one of which is the antioxidant compound content, namely flavonoids.
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Pages:10-14
How to cite this article:
Imrawati Imrawati, Yuri Pratiwi Utami, Ade Ainun Insani "Identification of compound content and antioxidant activity test of ethanol extract of <i>Stachytarpheta jamaicensis</i> (l.) vahl. leaf abts method". International Journal of Research in Pharmacy and Pharmaceutical Sciences, Vol 8, Issue 3, 2023, Pages 10-14
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