Background: Efficient extraction of bioactive compounds from medicinal plants is crucial for drug development. Traditional methods often face limitations such as low yields, high solvent consumption, and environmental concerns.

Objective: This study aims to develop and validate a novel ultrasonication-assisted extraction method to optimize bioactive compound recovery from Ocimum sanctum and Curcuma longa.

Methods: Plant powders were subjected to ultrasonication-assisted extraction using 70% ethanol. Extraction efficiency was assessed by measuring total flavonoid content (TFC), total phenolic content (TPC), and overall yield. Validation was conducted following ICH guidelines, evaluating parameters such as precision, accuracy, and robustness.

Results: Compared to Soxhlet extraction, the novel method increased TFC by 38.7% and 35.8% for Ocimum sanctum and Curcuma longa, respectively. Similarly, TPC improved by 40.8% and 40.2%, with overall yields rising by 4.2-5.5%. Validation results confirmed high reliability, with R² > 0.998 for linearity and accuracy between 95.8%-102.3%.