Objective: Aerva lanata has been used in traditional health systems to treat various disorders including as astringent, cooling, vermiuge, supparative, diuretic and lithontriptic. However, urolithiatic potential of leaves is not scientifically validated till date. The aim of present study is to evaluate urolithiatic effect of ethanol extracts of Aerva lanata leaves.

Methods: Ethylene glycol (EG) is used to induce urolithiasis in rats by adding \(0.75\%\) EG to their drinking water for several weeks, causing hyperoxalluria and the formation of calcium oxalate (CaOx) stones.

Results: Nearly equivalent to the standard drug, Cystone (70.58% inhibition), indicating a highly promising therapeutic potential. Comparable to the parent ethanolic leaf extract (66.15% inhibition), suggesting that the fractionation process successfully concentrated the anti-nucleation compounds within this specific fraction.