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VOL. 11, ISSUE 3 (2026)
Development and validation of LC–MS/MS method for the quantitative determination of Lacidipine in human plasma using solid-phase extraction
Authors
Dr. Ravi Teja Bandla, Ullemmagari Vineela
Abstract
A rapid, sensitive, and reproducible LC-MS
method was developed and validated for the quantitative determination of
lacidipine in human plasma using carbamazepine as the internal standard. Plasma
samples were processed by solid-phase extraction using Oasis HLB cartridges and
separated on a Zorbax Eclipse XDB-Phenyl column with acetonitrile and 5 mM ammonium
acetate (80:20, v/v) at a flow rate of 1.0 mL/min. Detection was performed
using positive electrospray ionization. The chromatographic run time was 2.5
min, enabling high-throughput analysis. The assay demonstrated linearity over
the concentration range of 0.2052–12.5026 ng/mL with a correlation coefficient
greater than 0.99 and an LLOQ of 0.2052 ng/mL. Validation studies confirmed
satisfactory selectivity, precision, accuracy, recovery, matrix effect,
dilution integrity, ruggedness, carry-over, and stability under all tested
conditions. Mean extraction recovery of lacidipine was approximately 91.9%, and
no significant endogenous interference or matrix effect was observed. The
proposed method is appropriate for routine pharmacokinetic, bioavailability, bioequivalence,
and therapeutic monitoring studies requiring rapid and reliable quantification
of lacidipine.
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Pages:42-47
How to cite this article:
Dr. Ravi Teja Bandla, Ullemmagari Vineela "Development and validation of LC–MS/MS method for the quantitative determination of Lacidipine in human plasma using solid-phase extraction". International Journal of Research in Pharmacy and
Pharmaceutical Sciences, Vol 11, Issue 3, 2026, Pages 42-47
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